Any video camera equipment has certain temporal resolution, so it will cause motion blur and motion aliasing in captured video sequence. Spatial deblurring and temporal interpolation are usually adopted to solve this problem, but these methods can not solve it completely in origin. A temporal super-resolution reconstruction method based on Maximum A Posterior (MAP) probability estimation for single-video was proposed in this paper. The conditional probability model was determined in this method by reconstruction constraint, and then prior information model was established by combining temporal self-similarity in video itself. From these two models, estimation of maximum posteriori was obtained, namely reconstructed a high temporal resolution video through a single low temporal resolution video, so as to effectively remove motion blur for too long exposure time and motion aliasing for inadequate camera frame-rate. Through theoretical analysis and experiments, the validity of the proposed method is proved to be effective and efficient.